The book entitled Handbook Of Plant Tissue Culture has been repared to meet the needs of students for biotechnology, Botany and Lifescience at undergraduate and post-graduate courses. The book cover all the important aspect of plant tissue culture for example micro propagation, callus culture, haploid culture, triploid culture, in vitro pollination, embryo culture, cell culture, protoplast culture and somatic hybridization, somaclonal variation, synthetic seed. in vitro secondary metabolite production and germplasm conservation Handbook Of Plant Tissue Culture, its requirement, basis and principles have been discussed in three independent chapters. In most of the chapter general methodology, diagrams, application, advantage and disadvantage of different aspects have been discussed.Different aspect are supplemented with photographs for easy understanding .The book will be found useful not only to the students but also research Scholars, teachers and other users wishig to embark upon plant Tissue Culture.